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Biostimulation of human chondrocytes with Ga-Al-As diode laser: ‘in vitro’ research

Morrone G, Guzzardella G A, Tigani D et al. PMID: 10728586 [PubMed – indexed for MEDLINE] Artif Cells Blood Substit Immobil Biotechnol. 2000 Mar;28(2):193-201   The aim of the study was to verify the effects of lllt performed with GaAlAs (780 nm, 2500 mW) on human cartilage cells in vitro. The cartilage sample used for the biostimulation treatment was taken from the right knee of a 19-year-old patient. After the chondrocytes were isolated and suspended for cultivation, the cultures were incubated for 10 days. The cultures were divided into four groups. Groups I, II, III were subject to biostimulation with the following laser parameters: 300J, 1W, 100Hz,10 min. exposure, pulsating emission; 300J, 1W, 300Hz, 10 min. exposure, pulsating emission; and 300J, 1W, 500Hz, 10 min. exposure, pulsating emission, respectively. Group IV did not receive any treatment. The laser biostimulation was conducted for five consecutive days. The data showed good results in terms of cell viability and levels of Ca and Alkaline Phosphate in the groups treated with laser compared to the untreated group. The results obtained confirm our previous positive in vitro results that the GaAlAs Laser provides biostimulation without cell damage.

Thermographic study of Low Level Laser Therapy for Acute-Phase Injury

Yoshimi Asagai, M.D.1, Atsuhiro Imakiire, M.D.2, Toshio Ohshiro, M.D.3, 1. Shinano Handicapped Children`s Hospital Shimosuwa, Nagano, Japan 2. Department of Orthopaedic Surgery, Tokyo Medical University Shinjuku, Tokyo, Japan 3. Japan Medical Laser Laboratory, Shinanomachi, Tokyo, Japan Presentation at World Association of Laser Therapy annual conference in Athens,Greece. 2000.   Acute-phase injury is generally treated by localized cooling of the region, and rarely by the active use of low level laser therapy (LLLT) in Japan. Thermographic studies of acute-phase injury revealed that circulatory disturbances at the site of trauma occurred due to swelling and edema on the day following the injury, and that skin temperature was high at the site of the trauma and low at the periphery. Following LLLT, circulatory disturbances rapidly improved, while temperature in the high temperature zone around the site of trauma fell by 3 degrees on the average, but at the periphery the low temperature rose by 3 degrees on the average to nearly normal skin temperature. Clinically, swelling and edema improved. LLLT was also useful in treating necrosis of the skin in the wound area and in accelerating healing of surgical wounds of paralytic feet, which are prone to delayed, wound healing and also wounds […]

Laser and Sports Medicine in Plastic and Reconstructive Surgery.

Junichiro Kubota M.D. Department of Plastic and Reconstructive Surgery, Kyorin University School of Medicine, Tokyo, Japan. Abstract from II Congress of the Internat. Assn for Laser and Sports Medicine, Rosario, Argentina, March 10-12, 2000.   Flap survival with diode laser therapy: Skin flap or graft surgery are major procedures in Plastic and Reconstructive Surgery. Skin flap necrosis has been a problem for us. The author reported on the enhanced blood flow following the low reactive laser therapy in skin flaps. The 830 nm diode laser (20 – 60 mw) irradiated flaps showed a greater perfusion, a greater number of blood vessels, and a higher rate of survival areas than the control flaps in the rat models and clinical cases. Improvement of wound healing with diode laser therapy: The diode laser therapy was indicated for traumatic skin ulcers received from sport activities and traffic accidents which had proved resistant to conservative treatment. The diode laser system has a wavelength of 830 nm. and output power of 150or 1000mw in continuous wave. The diode laser was applied with the non-contact method to the area on the wound for one minute once a day every day during the treatment period. The diode laser […]

What is optimal dose, power density and timing for low level laser therapy in tendon injuries? A review of in vitro and in vivo trials

J.M. Bjordal, C. Couppe University of Bergen, Dept. Physiotherapy Science, Bergen, Norway Abstract from the 7th International Congress of European Medical Laser Association, Dubrovnik, Croatia, June 2000. What is optimal dose, power density and timing for low level laser therapy in tendon injuries? A review of in vitro and in vivo trials   Purpose: To investigate the effect of different laser treatment parameters on fibroblast inflammation and production of collagen fibers. Material : Controlled in vitro or in vivo trials with low level laser therapy (LLLT) Method : Literature search for trials published after 1980 using LLLT on Medline, Embase, Cochrane Library and handsearch of physiotherapy journals in English and Scandinavian languages. Optimal treatment parameters regarding timing, treatment frequency, dose and power density at target tissue were synthesized. Results : The literature search identified 31 controlled trials with LLLT on collagen tissue. Three in vitro trials were performed on stretch-induced and inflammation in fibroblast cultures and five in vitro trials were performed on collagen production. Optimal dose and power density for inhibition of prostaglandin PGE2 and interleukin 1- beta production was found to be 3.2-6.3 J/cm2 and 5.3 mW/cm2 measured at the target fibroblast cells after 5 days of irradiation. […]

The Biological Effects of Laser Therapy and Other physical Modalities on Connective Tissue Repair Processes

Chukuka S. Enwemeka, P.T., Ph.D., FACSM, G. Kesava Reddy, Ph.D., Department of Physical Therapy and Rehabilitation Sciences, University of Kansas Medical Center, Kansas City, KS 66160-7601, USA The Journal of Laser Therapy. Vol. 12. World Association of Laser Therapy. 2000   Connective tissue injuries, such as tendon rupture and ligamentous strains, are common. Unlike most soft tissues that require 7-10 days to heal, primary healing of tendons and other dense connective tissues take as much as 6 – 8 weeks during which they are inevitably protected in immobilization casts to avoid re-injury. Such long periods of immobilization impair functional rehabilitation and predispose a multitude of complications that could be minimized if healing is quickened and the duration of cast immobilization reduced. In separate studies, we tested the hypothesis that early function, ultrasound, 632.8 nm He-Ne laser, and 904 nm Ga-As laser, when used singly or in combination, promote healing of experimentally severed and repaired rabbit Achilles tendons as evidenced by biochemical, biomechanical, and morphological indices of healing. Our results demonstrate that: (1) appropriate doses of each modality, i.e., early functional activities, ultrasound, He-Ne and Ga-As laser therapy augment collagen synthesis, modulate maturation of newly synthesized collagen, and overall, enhance the […]